Optimization of In Vitro Propagation of Pear (Pyrus communis L.) ‘Pyrodwarf®(S)’ Rootstock

نویسندگان

چکیده

Pears are among the most economically important fruits in world that grown all temperate zones. Pyrus communis L., ‘Pyrodwarf®(S)’ rootstock is one of gene sources used to improve fruit productivity, resistance, and tolerance biotic abiotic stresses. Traditional propagation P. L. time-consuming limited by a short growing season harsh winter conditions. Therefore, vitro suitable alternative. Murashige Skoog medium (MS) woody plant (WPM) supplemented with different concentrations 6-benzyladenine (BA) kinetin (Kin), individually or combination, were for shoot proliferation. Nodal segments as explants. MS augmented indole-3-butyric acid (IBA) indole-3-acetic (IAA) was then rooting microshoots. A combination 2 mg·L?1 BA 1 Kin resulted significant improvement This produced highest number shoots (4.352 per explant) leaves (10.02 explant). The longest (4.045 cm) obtained WPM enriched BA. However, these not multiplication steps. largest roots (5.50 microshoot) on IAA at mg·L?1. plantlets cultivated pots filled perlite cocopeat (in ratio 1:3) acclimatized gradually greenhouse, recording an even 90% survival rate.

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ژورنال

عنوان ژورنال: Agronomy

سال: 2023

ISSN: ['2156-3276', '0065-4663']

DOI: https://doi.org/10.3390/agronomy13010268